A record five Americas teams will compete at Rugby World Cup 2027. The Australia hosted tournament will be an expanded tournament with 24-teams rather than 20 as was the case from 1999-2023. The five Americas teams will be Canada and the USA from North America and Argentina, Chile and Uruguay from South America.
As is the case in international rugby competition, national anthems are a key component of match-day activities. The five anthems have all been performed at past Rugby World Cups. They are displayed below.
| CANADA |
 USA |
 URUGUAY |
 ARGENTINA |
 CHILE |